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anti bcl 2  (Proteintech)


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    Structured Review

    Proteintech anti bcl 2
    Anti Bcl 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcl+2/BCL2+Fusion+Protein/pmc12969010-181-13-11
    Average 99 stars, based on 194 article reviews
    anti bcl 2 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: CSF1 regulates inflammation and apoptosis in intervertebral disc degeneration.
    Article Snippet: .. The expressions of CSF1, GAPDH, TNF-α, BAX, Bcl-2, cleaved Caspase-3and cleaved Caspase-3 (Proteintech, Wuhan, China) were detected by Western blotting. ..

    Blocking Assay:

    Article Title: Dietary semen cuscuta improves laying performance by stabilizing mitochondria-associated membranes (MAMs) and inhibiting granulosa cell apoptosis in laying hens
    Article Snippet: .. The membranes underwent blocking with 5% skimmed milk diluted in PBS, and afterwards incubated employing primary antibodies against β-actin (GB15001, Servicebio, Wuhan, China), IP3R (GB11742, Servicebio, Wuhan, China), GRP75 (GB11852, Servicebio, Wuhan, China), VDAC1 ( GB111939 , Servicebio, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Bax (50599-2-Ig, Proteintech, Wuhan, China), Cyt c (WL02410, Wanleibio, Shenyang, China), Cleaved Caspase-3 (WL01992, Wanleibio, Shenyang, China), GPR41 ( OM184469 , Omnimabs, California, United States), GPR43 ( OM255320 , Omnimabs, California, United States), Occludin (WL01996, Wanleibio, Shenyang, China) and ZO-1(21773-1-AP, Proteintech, Wuhan, China) overnight at 4°C with gentle shaking. ..

    Incubation:

    Article Title: Dietary semen cuscuta improves laying performance by stabilizing mitochondria-associated membranes (MAMs) and inhibiting granulosa cell apoptosis in laying hens
    Article Snippet: .. The membranes underwent blocking with 5% skimmed milk diluted in PBS, and afterwards incubated employing primary antibodies against β-actin (GB15001, Servicebio, Wuhan, China), IP3R (GB11742, Servicebio, Wuhan, China), GRP75 (GB11852, Servicebio, Wuhan, China), VDAC1 ( GB111939 , Servicebio, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Bax (50599-2-Ig, Proteintech, Wuhan, China), Cyt c (WL02410, Wanleibio, Shenyang, China), Cleaved Caspase-3 (WL01992, Wanleibio, Shenyang, China), GPR41 ( OM184469 , Omnimabs, California, United States), GPR43 ( OM255320 , Omnimabs, California, United States), Occludin (WL01996, Wanleibio, Shenyang, China) and ZO-1(21773-1-AP, Proteintech, Wuhan, China) overnight at 4°C with gentle shaking. ..

    Article Title: The Dual Role of Gastrodin in Spinal Cord Injury: Microglial Phenotype Switching and Neuronal Survival via PI3K/AKT Activation.
    Article Snippet: .. The membranes were blocked with 5% non- fat milk for 1 h at room temperature and then incubated overnight at 4°C with the following primary antibodies: β- Actin (1:5000, 20,536–1- AP, Proteintech), Arg- 1 (1:2000), iNOS (1:2000), Phospho- PI3 Kinase p85 (Tyr467)/p55 (Tyr199) (1:1000, ab278545, Abcam), total PI3 Kinase p85 (1:4000, 60,225–1- Ig, Proteintech), Phospho- AKT (Ser473) (1:2000, 28,731– 1- AP, Proteintech), total AKT (1:5000, 10,176–2- AP, Proteintech), Bcl- 2 (1:4000, 26,593–1- AP, Proteintech), Bax (1:4000, 50,599–2- Ig, Proteintech), Cleaved Caspase- 3 (1:1000, 9661 T, CST). .. After incubation with HRP- conjugated secondary antibodies (1:5000, Proteintech) for 1 h at room temperature, protein bands were visualized using an ECL kit (Proteintech) and imaged with a ChemiDoc imaging system (Bio- Rad).

    Article Title: Protective Effect of Silymarin Against Paclitaxel‐Induced Cardiotoxicity
    Article Snippet: .. The membranes were blocked with 5% w/v bovine serum albumin for 90 min. Primary antibodies (IL‐1β [sc‐52,012], TNF‐α [sc‐52,746], Caspase‐3 [sc‐56,053], Bcl‐2 [sc‐7382], NF‐κB‐p65 [sc‐109], and beta‐actin [sc‐47,778], Santa Cruz Biotechnology, USA; P2X7R [11144–1‐AP], Proteintech Group, USA) were applied to the membranes and incubated at 4°C for approximately 15 h. Following a wash with Tris‐buffered saline containing Tween, the membranes were exposed to secondary antibodies (sc‐2004/sc‐2005, Santa Cruz Biotechnology, USA) conjugated with horseradish peroxidase (TP‐125‐HL, Thermo Fisher Scientific, USA) for 90 min. β‐actin (1:2000 dilution) was used as the loading control. .. Protein bands were visualized using enhanced Western ECL substrate (3405, Thermo Fisher Scientific, USA) and analyzed with software (Image Lab, Bio‐Rad, USA).

    Gentle:

    Article Title: Dietary semen cuscuta improves laying performance by stabilizing mitochondria-associated membranes (MAMs) and inhibiting granulosa cell apoptosis in laying hens
    Article Snippet: .. The membranes underwent blocking with 5% skimmed milk diluted in PBS, and afterwards incubated employing primary antibodies against β-actin (GB15001, Servicebio, Wuhan, China), IP3R (GB11742, Servicebio, Wuhan, China), GRP75 (GB11852, Servicebio, Wuhan, China), VDAC1 ( GB111939 , Servicebio, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Bax (50599-2-Ig, Proteintech, Wuhan, China), Cyt c (WL02410, Wanleibio, Shenyang, China), Cleaved Caspase-3 (WL01992, Wanleibio, Shenyang, China), GPR41 ( OM184469 , Omnimabs, California, United States), GPR43 ( OM255320 , Omnimabs, California, United States), Occludin (WL01996, Wanleibio, Shenyang, China) and ZO-1(21773-1-AP, Proteintech, Wuhan, China) overnight at 4°C with gentle shaking. ..

    other:

    Article Title: Protective effect of Zymosan-A against radiation-induced premature ovarian insufficiency in a murine model
    Article Snippet: BCL-2 , Proteintech , Cat# 60178-1-Ig; RRID: AB_10734459.

    Saline:

    Article Title: Protective Effect of Silymarin Against Paclitaxel‐Induced Cardiotoxicity
    Article Snippet: .. The membranes were blocked with 5% w/v bovine serum albumin for 90 min. Primary antibodies (IL‐1β [sc‐52,012], TNF‐α [sc‐52,746], Caspase‐3 [sc‐56,053], Bcl‐2 [sc‐7382], NF‐κB‐p65 [sc‐109], and beta‐actin [sc‐47,778], Santa Cruz Biotechnology, USA; P2X7R [11144–1‐AP], Proteintech Group, USA) were applied to the membranes and incubated at 4°C for approximately 15 h. Following a wash with Tris‐buffered saline containing Tween, the membranes were exposed to secondary antibodies (sc‐2004/sc‐2005, Santa Cruz Biotechnology, USA) conjugated with horseradish peroxidase (TP‐125‐HL, Thermo Fisher Scientific, USA) for 90 min. β‐actin (1:2000 dilution) was used as the loading control. .. Protein bands were visualized using enhanced Western ECL substrate (3405, Thermo Fisher Scientific, USA) and analyzed with software (Image Lab, Bio‐Rad, USA).

    Control:

    Article Title: Protective Effect of Silymarin Against Paclitaxel‐Induced Cardiotoxicity
    Article Snippet: .. The membranes were blocked with 5% w/v bovine serum albumin for 90 min. Primary antibodies (IL‐1β [sc‐52,012], TNF‐α [sc‐52,746], Caspase‐3 [sc‐56,053], Bcl‐2 [sc‐7382], NF‐κB‐p65 [sc‐109], and beta‐actin [sc‐47,778], Santa Cruz Biotechnology, USA; P2X7R [11144–1‐AP], Proteintech Group, USA) were applied to the membranes and incubated at 4°C for approximately 15 h. Following a wash with Tris‐buffered saline containing Tween, the membranes were exposed to secondary antibodies (sc‐2004/sc‐2005, Santa Cruz Biotechnology, USA) conjugated with horseradish peroxidase (TP‐125‐HL, Thermo Fisher Scientific, USA) for 90 min. β‐actin (1:2000 dilution) was used as the loading control. .. Protein bands were visualized using enhanced Western ECL substrate (3405, Thermo Fisher Scientific, USA) and analyzed with software (Image Lab, Bio‐Rad, USA).



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    Image Search Results


    The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

    Journal: Poultry Science

    Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

    doi: 10.1016/j.psj.2026.106922

    Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

    Article Snippet: Bcl2 Rabbit Ab , Bioss , bs-0032R , 1: 1500.

    Techniques: Expressing

    The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

    Journal: Poultry Science

    Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

    doi: 10.1016/j.psj.2026.106922

    Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

    Article Snippet: Bcl2 Rabbit Ab , Bioss , bs-0032R , 1: 1500.

    Techniques: Activity Assay